High-Efficiency SS320 Competent Cells for Phage Display

The E. coli strain SS320 (also known as MC1061F′) is a non-amber suppressor strain prepared as high transformation efficiency (≥ 1 × 10^9 cfu/μg) chemically competent cells. It is specifically optimized for robust phage display library screening and constructing large antibody or protein libraries.

Key Features & Benefits

• High Transformation Efficiency: > 1 x 10^9; transformants / μg pBR322, ideal for high-throughput library screening.
• F′ Pilus Maintenance: Harbors an episome containing the F′ pilus required for bacteriophage entry, allowing seamless amplification of phage libraries.
• Tetracycline Resistant: Contains the Tn10 (tetR) gene; cells should be grown with tetracycline to maintain the F′ pilus.
• Fast-Growing Strain: Accelerates cloning, biopanning, and protein expression workflows.

Product Specifications

• Genotype: hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galU galK rpsL thi [F´ proAB+ lacI¹ lacZΔM15 Tn10 (tetr)]
• Applications: Peptide phage display, antibody library preparation, biopanning, and protein expression.
• Storage Conditions: Store at -80°C. Ships on dry ice.

Catalog # SS320-201

This product is intended for research use only.